Preservation of Spermatozoa Sumba Ongole Bulls Using Citrate Yolk Diluent with the Addition of Palmyra Palm Juice
DOI:
https://doi.org/10.30736/jt.v11i2.83Keywords:
Semen, Egg Yolk Citrate, palm sap, preservation, sumba ongoleAbstract
The purpose of this study was to determine the quality of the ability of spermatozoa cells to maintain the use of a yolk citrate (YC) diluent with the addition of palmyra palm juice during preservation. The material used for this research was 2 Sumba Ongole bulls as a source of semen. Semen was collected by using an artificial vagina that is processed twice a week. The obtained semen was macroscopically analyzed, including volume, colour, consistency, pH and smell. Meanwhile, microscopic evaluation includes mass movement, motility, concentration, viability and abnormalities. The semen showed >75% motile sperm divided into four treatments and dilute each with YC (P0), 95% YC+5% palmyra palm juice (PPJ) (P1), 90% YC+10% PPJ (P2), 85% YC+15% PPJÂ and each was stored at 3-50C. The results of statistical analysis showed that P0, P1 treatments were significantly different (P<0.05) between P2, P3 from day 0 to day 4 of storage. The addition of palmyra palm did not affect the motility of spermatozoa, but had a positive effect on the viability of spermatozoa with the addition of 5% palm sap during storage for 4 days at a temperature of 3-5 0C.
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Azzahra, F.Y, Setiatin, E.T, dan Samsudewa, D, “Evaluation of Sperm Motility and Viability of Fresh Semen of Kebumen PO Bulls,†Jurnal Sain Peternakan Indonesia, vol 11, no. 2, pp. 99-107, 2016.
Affandhy L., Luthfi M., Ratnawati D., Firdaus F, “The Effect of Moringa oleifera (MO) Leaf Powder on the Quantity and Quality of Semen of Ongole Crossbreed,†Jurnal of Tropical Animal Science and Technology, vol 7, no. 2, pp. 119-127, 2020.
Danang D., Isnaini N., dan Trisunuwati P, “The Effect of Storage Time on Native Chikens Spermatozoa Quality by Ringer Solution Diluent in 4 0C Temperature, “ Jurnal Ternak Tropika, vol. 13, no.1, pp. 47–57, 2012.
Fatkhul Mubin, M., Zubaidah, E., Kunci, K, â€Studi Pembuatan Kefir Nira Siwalan (Borassus flabellifer l.) (Pengaruh Pengenceran Nira Siwalan dan Metode Inkubasi),†Jurnal Pangan dan Agroindustri, vol. 4 no.1, pp. 291–301, 2016.
Garner DL, Hafez ESE, “Spermatozoa and Seminal Plasma. Reproduction In Farm Animal 7th Eds. Edited By Hafez ESE, Hafez, B. Baltimore,†Lippincott & Williams, ed. 7, pp. 96- 109, 2008.
Hafez ESE, Hafez B, â€Preservation and Cryopreservation Of Gamete And Embryos. Reproduction In Farm Animals. 7th Eds. Edited By Hafez ESE, Hafez, B. Baltimore,†Lippincott Williams and Wilkins ed. 6, pp. 82-95, 2008.
Heriyanta E., Ihsan M. N., Isnaini N, “Pengaruh Umur Kambing Peranakan Etawa (PE) Terhadap Kualitas Semen Segar,†Jurnal Ternak Tropika, vol. 14 no.2, pp. 1-5, 2013.
Ismaya, “Bioteknologi Inseminasi Buatan pada Sapi dan Kerbau,†Yogyakarta, Gadjah Mada University Press, 2014.
Kusumawati E. D, Krisnaningsih A. T. N, Ramadlon R. R, “Kualitas Spermatozoa Semen Beku Sapi Simental dengan Suhu dan Lama Thawing yang Berbeda,†Jurnal Ilmu-Ilmu Peternakan, vol. 26, no. 3, pp. 38-41, 2016.
A Kusumawati E.D, Betu H, Krisnaningsih A.T.N, Rahadi S, “Kualitas Semen Segar Sapi Limousin Pada Lama Simpan yang Berbeda†Jurnal Ilmiah Fillia Cendekia, vol. 3, no. 1, pp. 1-9, 2018.
A Pareira G. R., Becker E. G., Siquiera L.C., Ferreira R., Severo C. K., Truzzi V. S., Oliveira J. F. C. and Goncalves P. B. D, “Assessment Of Bovine Spermatozoa Viability Using Different Cooling Protocols Prior To Cryopreservation,†Italian Journal Of Animal Science, vol. 9, pp. 403- 407, 2010.
A Pubiandara S., Suharyati S., Hartono M, “Pengaruh Penambahan Dosis Rafinosa dalam Pengencer Sitrat Kuning Telur Telur terhadap Motilitas Persentase Hidup dan Abnormalitas Spermatozoa Sapi Ongole,†Jurnal Ilmu Peternakan Terpadu, vol. 4, no.4, pp. 292-299, 2016.
A Rahmawati M. A., Susilawati T., Ihsan M. N, “Kualitas Semen dan Produksi Semen Beku pada Bangsa Sapi dan Bulan Penampungan yang Berbeda,†Jurnal Ilmu-Ilmu Peternakan, vol. 25, no. 3, pp. 25-36, 2014.
A Sukmawati E, R.I Arifiantini dan B Purwantara, “Daya Tahan Spermatozoa terhadap Proses Pembekuan pada Berbagai Jenis Sapi Pejantan Unggul,†Jurnal Veteriner, vol. 19, pp. 168-175, 2014.
A Sulistyowati D., Faris M. A., Yekti A. P. A, “Kualitas Semen Cair Sapi Peranakan Ongole pada Pengencer Tris Aminomethane Kuning Telur Tanpa Raffinose yang Disimpan Pada Media yang Berbeda Suhu,†Jurnal Ternak Tropika, vol. 19, no. 1, pp. 38-45, 2018.
A Susilawati, T, “Tingkat Keberhasilan Inseminasi Buatan dengan Kualitas dan Deposisi Semen yang Berbeda pada Sapi Peran-Akan Ongole,†Jurnal Ternak Tropika, vol. 12, no. 2, pp. 15-24, 2011.
A Susilawati, “Pedoman Inseminasi Buatan Pada Ternak,†UB Press, Universitas Brawijaya, 2013.
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