Antiviral Potential of Patikan Kebo Extract against Chicken Corona Virus through In Ovo Challenge Test
DOI:
https://doi.org/10.30736/jt.v14i2.174Keywords:
Virus, Corona, Patikan Kebo, IB, ChickenAbstract
The coronavirus, known to exist in humans and livestock for an extended period, includes variants such as the infectious bronchitis (IB) virus in chickens. As a single-stranded RNA virus, coronaviruses are prone to mutations. Patikan Kebo (Euphorbia hirta L) is a medicinal herb containing various compounds like Flavonoids, Mirisil, Alkaloids, Laraserol, Hentriakontanol, and Komositin, known for their antibacterial, antiviral, and anti-worm properties. This study aimed to evaluate the toxicity of Patikan Kebo extract on Chicken Embryo Eggs (TAB) and assess its antiviral potential against the chicken AI coronavirus. The experimental approach involved three concentrations of Patikan Kebo extract (0.01% - P1, 0.1% - P2, 1% - P3), a control group without Patikan Kebo extract (K+), and a control group without the virus (K-). Data analysis was conducted using ANOVA and Tukey HSD in the SPSS program. The results from ANOVA indicated that the dose of Patikan Kebo significantly impacted (P<0.05) its antiviral activity against the IB virus. The average HA Titer 2 (Log2) demonstrated a 47% reduction in viral activity. In conclusion, the most effective herbal antiviral dose against the IB virus was determined to be 0.1% Patikan Kebo extract.
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References
Ministry of Health, "Corona Virus Mutations Spread Faster, People Are Called to Tighten Health Protocol Discipline,"www. kemkes.go.id2020
A. Susiloet al., “Review of Current Literatures Mutations and Variants of Coronavirus Disease 2019 (COVID-19): A Review of Recent Literature,†J. Indonesian Internal Medicine, vol. 9, no. 1, pp. 59–81, 2022.
NLP Indi Dharmayanti and R. Indriani, “Identification and Characterization of Infectious Bronchitis Virus (IBV) in Indonesia,â€J. Biol. Indonesia., vol. 13, no. 1, pp. 53–60, 2017, doi:10,47349/jbi/13012017/53.
NS Yuliani and GYI Sakan, "Antibody Titer Responses in Broiler Chickens Vaccinated with Nd and Given Herbs,"Partners, vol. 23, no. 2, p. 696, 2018, doi:10.35726/jp.v23i2.312.
“66366-EN-benefits-extract-ethanol-patikan-kebo-euph.pdf.â€
Nurhayati, Nelwilda, and H. Handoko, "Addition of Medicinal Weeds in The Ration on Broiler Chicken Performance,"anime. Prod., vol. 11, no. 2, pp. 103–108, 2020.
E. Hirta, LT Total, Y. Infected, and D. Eimeria, “Hemoglobin Levels, and Hematocrit Values in Chickens,†no. 1992, pp. 126–133, 2020.
AM Hassan, “Effect of in Ovo injection with Nano- Selenium or Nano- Zinc on Post-Hatch Growth Performance and Physiological Traits of Broiler Chicks,â€int. J. Environ. agric. Biotechnol., vol. 3, no. 2, pp. 350–357, 2018, doi:10.22161/ijeab/3.2.6.
Z. Caoet al., “Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo,†Proteome Sci., vol. 10, no. 1, 2019, doi:10.1186/1477-5956-10-24.
EK Wabalo, AD Dubiwak, MW Senbetu, and TS Gizaw, “Effect of genomic and amino acid sequence mutation on virulence and therapeutic target of severe acute respiratory syndrome coronavirus-2 (Sars cov-2),â€infected. Drug Resistant., vol. 14, pp. 2187–2192, 2021, doi:10.2147/IDR.S307374.
YT Wulandari K. E, R. Susanti, and SH Bintari, "Analysis of Antibody Titer Development Resulting from Infectious Bronchitis Vaccination in Layers of Hisex Brown Strain,"Life Science., vol. 8, no. 1, pp. 25–33, 2019, doi:10.15294/lifesci.v8i1.29987.
BI Biology, “Biosantifika,†vol. 4, no. 2, 2012.
Istikomah and TBSA,"The Effectiveness of the H5N1 Subtype Avian Influenza Vaccine on Kampung Chicken in Legok, Tangerang, Banten†vol. 12, no. 2007, pp. 703–712, 2014, [Online]. Available: https://hsgm.saglik.gov.tr/depo/birimler/saglikli-beslenme-hareketli-hayat-db/Yayinlar/kitaplar/diger-kitaplar/TBSA-Beslenme-Yayini.pdf
V. Tetelo, B. Microbiology, Animal Medicine Faculty, and UG Mada, "Isolation, Identification, Physical Properties, and Biology," vol. 13, no. 4, pp. 425–433, 2012.
DC Fiandri and Sutarto, "The Potential of the Patikan Kebo (Euphoria Hirta) Plant as a Wound Healer,"J. Med. Main, vol. 02, no. 01, pp. 224–230, 2020.
PDAE Al-Snafi, “Pharmacology and therapeutic potential of Euphorbia hirta (Syn: Euphorbia pilulifera)- A review,â€IOSR J. Pharm., vol. 07, no. 03, pp. 07–20, 2017, doi: 10.9790/3013-0703010720.
GR Teodoro, K. Ellepola, CJ Seneviratne, and CY Koga-Ito, “Potential use of phenolic acids as anti-Candida agents: A review,â€Front. Microbiol., vol. 6, no. DEC, pp. 1–11, 2015, doi:10.3389/fmicb.2015.01420.
PT Wina, "Effect of Sapindus rarak flour as a feed additive on the performance and lipid profile of broiler chickens infected with Eimeria tenella,"J. Animal Science and Vet., vol. 19, no. 4, pp. 263–271, 2020.
M. Kopjar, M. Orsolic, and V. Pilizota, “Anthocyanins, phenols, and antioxidant activity of sour cherry puree extracts and their stability during storage,â€int. J. Food Prop., vol. 17, no. 6, pp. 1393–1405, 2014, doi:10.1080/10942912.2012.714027.
W. Ibrahim, R. Mutia, Nelwida, and Berliana, "Use of Fermented Pineapple Peel in Ration Containing Weeds with Medicinal Properties Against the Consumption of Broiler Chicken Nutrients (Fermented pineapple peel supplementation with addition of medicinal weeds on nutrient intake consumption of broiler chicken),"Agrippet., vol. 16, no. 2, pp. 76–82, 2016.
Y. Mariani and MA Hamzani, "INFLUENCE OF HATCHING TEMPERATURE ON FERTILITY, MORTALITY AND HATABILITY OF VILLAGE CHICKEN EGGS (Gallus domesticus) IN INCUBATORS,"AGRIPTEK (Journal of Agribusiness and Livestock), vol. 1, no. 1, pp. 23–28, 2021, doi:10.51673/agriptek.v1i1.611.
ER Yuslianti, B. Bachtiar, D. Fatma Suniarti, and A. BSutjiatmo, “Pharmaceutical Standardization of Natural Ingredients Towards Phytopharmaceuticals for the Development of Indonesian Traditional Medicines (NaturalProducts Pharmaceutical Standardization Towards Phytopharmaca for Indonesian Traditional Medicine Development),†J. Dentika, vol. 19, no. 2, pp. 179–185, 2016.
GN Aziz, S. Suwarno, RN Praja, J. Rahmahani, PA Wibawati, and F. Fikri, "Identification of the Development of Infectious Bronchitis Virus Isolate Local And Massachusetts In Allantois Fluid TAB Using Indirect Fluorescence Antibody Technique,"J. Med. Vet., vol. 2, no. 1, p. 18, 2019, doi:10.20473/jmv.vol2.iss1.2019.18-23.
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